Nephroprotective activities of antioxidants of Couroupita guianensis Aubl flowers extract against chloramphenicol induced nephrotoxihcity in Mice

Abstract

Development of remedies providing protection from chloramphenicol induced oxidative stress and nephron-toxicity would be the nice clinical significance. The present study was designed to investigate nephroprotective activities of antioxidants of Couroupita guianensis Aubl flowers extract on chloramphenicol induced nephrotoxicity in Mice. Twenty four Swiss Albino mice were divided into four equal groups as follows: I-Control group, II-Toxic group, Chloramphenicol 500 mg/kg body weight (oral); III- Extract group, 200 mg/kg body weight (oral) FE of CG; IV-Prophylactic group, Chloramphenicol 500 mg/kg (oral) + 200 mg/kg (oral) FE of CG. The treatments were administered once in a day for 14 days. It is followed by one day fasting to the mice. After 15 days biochemical and histopathological analysis were conducted to evaluate oxidative stress and nephrotoxicity. Serum level of blood urea nitrogen (BUN), creatinine, superoxide dismutase (SOD), catalase (CAT), total glutathione (GSH) and lipid peroxidase (LPO) were evaluated. The mice treated with chloramphenicol alone showed increase in BUN and LPO, while the level of creatinine, SOD, CAT and GSH were declined. The treatment of mice with FE of CG showed improvement of kidney function, as a result of decreased biochemical indices and oxidative stress parameters associated with chloramphenicol induced nephron-toxicity. The histopathological examination of mice kidneys showed slight improvement in the structure. Hence the natural antioxidants of FE of CG may protect the kidney against chloramphenicol induced nephro-toxicity and oxidative stress in mice.   It is concluded that the antioxidants in the FE of CG has swietenine, sapropterin, usnic acid, lupeol and gamma tocopherol (vit. E) etc are useful to share the electron with free radicals in order to stop the role of free radicals to damage the kidney.