Isolation of Pseudomonas aeruginosa and testing of lipase (EC 3.1.1.3) production conditions.

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Ayat Ahmed Ali, Khalid W. Hameed, Mohammed I. Nadder

Abstract

Pseudomonas aeruginosa isolates were obtained from soil contaminated with petroleum oils and
cultured in different nutrient media. The appropriate isolate was chosen for the production of
lipase enzyme and the change in production conditions was carried out as temperature, where the
maximum activity of lipase enzyme reached (3U/ml) at a temperature of 37 degrees Celsius,
while the pH was the best activity (3.2U/ml) at pH 7 and the change of carbon sources reached
the maximum activity of the enzyme (2.9U/ml) at the use of glucose as a carbon source, while at
Nitrogen sources, the maximum enzyme activity (3.5U/ml) was reached when using peptone as a
source. The lipase produced from the soil will be useful in increasing industrial production.

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